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    Forensics

    Microreader™ 21 ID System

    Microreader™ 21 ID System

    The Microreader™ 21 ID System is a 5-dye direct-amplification STR kit that amplifies 20 STR loci (including 13 CODIS loci) as well the highly discriminating Penta D and Penta E loci and a gender marker: Amelogenin for maximum concordance with existing kits and high confidence in paternity and relationship testing conclusions. 


    For supplement autosome, X-chromosome and Y chromosome testing kits, please see "Microreader™ 23sp ID System", "Microreader™ 19X ID System".

    Introduction

    The Microreader™ 21 ID System is a 5-dye direct-amplification STR kit that amplifies 20 STR loci (including 13 CODIS loci) as well the highly discriminating Penta D and Penta E loci and a gender marker: Amelogenin for maximum concordance with existing kits and high confidence in paternity and relationship testing conclusions.

    The Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements.


    Key Features

    Classical commercial kit which has been used for more than 10 years domestically and worldwide.
    ■ Allows direct amplification from these common sample types used in paternity testing without the need for extraction or purification, such as blood and buccal samples deposited on swab or paper substrates, as well as extracted DNA from various sample.
    ■ Superior discrimination ability, with cumulative non-father exclusion rate CPE>0.999999995.
    ■ Improved genotyping accuracy through superior primer design, expanded allelic ladder and virtual bins.

    Superior discrimination performance

    It conforms to international standards and the forensic scientific DNA database construction specifications, has a high individual recognition rate, and its cumulative non-father exclusion rate is CPE>0.999999995, which is suitable for kinship testing, DNA database construction, and other forensic analysis. 

    High quality reproductivity

    This kit enables high-quality direct PCR amplification of reference samples with an improved first-pass success rate. You can expect:
    ① Well-resolved peaks above the background noise level
    ② Minimal occurrence of allelic drop-out, off-ladder allele calls, and off-scale data
    ③ Well-balanced profile within each dye color for maximized Intracolor Balance (ICB) values

    Direct amplification & higher efficiency

    Without the need for sample extraction, purification and quantification, the entire process from direct amplification of the samples to the PCR product can be completed in 90 minutes, which improves the work efficiency and reduced testing costs, which are very suitable for the processing of large batches of samples. 

    Anti-pollution design

    The kit has an anti-pollution design. When performing STR typing it can remove other PCR products that may be mixed in the amplification tube, avoid personnel contamination or non-specific amplification, and effectively reduce the impact of laboratory aerosol contamination. Increase the success rate of the experiment.

    Kit related published paper

    [1] Wu, Heming, et al. "Invasive molecular prenatal diagnosis of alpha and beta thalassemia among Hakka pregnant women." Medicine 97.52 (2018). (DOI: 10.1097/MD.0000000000013557)
    [2] Yang, Yaran, et al. "Haplotypic polymorphisms and mutation rate estimates of 22 Y-chromosome STRs in the Northern Chinese Han father–son pairs." Scientific reports 8.1 (2018): 1-6. (DOI: 10.1038/s41598-018-25362-3)

    Product Details

    Microreader™ 21 ID System Product Details

    For Use with (Application)

    Sequencing

    Unit size

    100/200 Reactions

    Label or Dye

    5 Dye

    Product type

    PCR amplification Kit

    Detection method

    Capillary Electrophoresis

    Shipping Condition

    Dry ice

    Stroge Condition

    Store at –25 to –15°C upon receipt and at 2–8°C after initial use

    Target Loci

    D19S433, D5S818, D21S11, D18S51, D6S1043, AMEL, D3S1358, D13S317, D7S820, D16S539, CSF1PO, PENTA D, D2S441, VWA, D8S1179, TPOX, PENTA E, TH01, D12S391, D2S1338, FGA