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    Forensics

    Microreader™ Y Prime ID System

    Microreader™ Y Prime ID System

    The Microreader™ Y Prime ID System is a 6-dye system that amplifies 29 Y-STR loci, which allows amplification from multiple male-specific sample types. This kit is designed for amplification in difficult cases such as degraded DNA samples or contacted stains, with higher sensitivity and stability. 


    For casework autosome kit, please see "Microreader™ 28A ID System". 

    For more Y-chromosome kits, please see "Microreader™ Y Prime Plus ID System".

    Introduction

    Microreader™ Y Prime ID system is designed for amplification in difficult cases such as degraded DNA samples or contacted stains using advanced 6-color fluorescent labeling technology, with higher sensitivity and stability. It is compatible with the commonly used commercial Y chromosome case detection kits, suitable for the identification of suspects in caseworks.

    The Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements.



    Key Features

    ■ Highly Sensitive: complete STR profiles could be obtained when the DNA template content was as low as 0.0625ng.
    ■ Improved performance for mixed sample detection, such as male-male and male-female mixed samples.
    ■ Improved performance with degraded samples and PCR inhibitors.

    Highly Sensitive

    By optimizing the PCR buffer system and thermal cycling parameters, the sensitivity of the kit was significantly improved, and the complete STR profiles could be obtained when the DNA template content was as low as 0.0625ng. 

    Superior discrimination power

    This kit is optimized to help improve resolution of trace evidence and sexual assault mixtures that contain small quantities of male DNA in a large background of female DNA. Also, for complex male-male mixed samples in the case, when the ratio is 1:4, complete STR profiles can be obtained; when the ratio is as high as 1:9, more than 97% of STR profiles can still be obtained. 

    Stronger ability of inhibition resistance

    It has higher anti-PCR inhibition ability and higher amplification efficiency for samples containing DNA inhibitors such as humic acid, hematin and tannic acid. When the concentration of hematin is 100uM and the humic acid concentration is 400ng/ul, complete STR profiles can still be obtained.

    Suitable for degraded samples

    10 mini-STR (<220bp) could be detected by specifically designed STR loci arrangement, which are very powerful when dealing with degraded samples.

    Kit related published paper

    Zhao, Yi, et al. "Validation of the Microreader™ 29Y Prime ID system for forensic use." Forensic science international 309 (2020): 110147. (DOI: 10.1016/j.forsciint.2020.110147.)

    Product Details

    Microreader™ Y Prime ID System Product Details

    For Use with (Application)

    Sequencing

    Unit size

    100/200 Reactions

    Label or Dye

    6 Dye

    Product type

    PCR amplification Kit

    Detection method

    Capillary Electrophoresis

    Shipping Condition

    Dry ice

    Stroge Condition

    Store at –25 to –15°C upon receipt and at 2–17°C after initial use

    Target Loci

    DYS570, DYS549, DYS546, DYS460, DYS458, DYS635, DYS533, DYS448, DYS627, DYS456, DYS576, DYS449, DYS437, DYS643, DYS518, DYF387S1 a/b, Y GATA H4, DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS438 and DYS439